Last updated: September 26, 2026 · Originally published: March 21, 2026 · By Eternal Elixir Editorial
Quick answer: No study has tested NMN and methylene blue together, and no interaction between the two has been reported. The safety question that matters is methylene blue on its own: it must not be combined with SSRI, SNRI or MAOI antidepressants or other serotonergic medicines, and it is not for people with G6PD deficiency, in pregnancy or breastfeeding, or under 18. If none of that applies to you, our stack is Eternal Elixir NMN 500mg (one capsule a day, $0.67) with Eternal Elixir Methylene Blue 10mg capsules (one a day, $1.00), which comes to $1.67 a day. Talk to your doctor first if you take any prescription medicine.
Eternal Elixir makes both products in this stack. This guide explains what each one does, what the human studies show (and what they don’t), how to take them, and who should not. Every research claim links to the published study.
Who should not take methylene blue
Read this before anything else on this page. Do not take methylene blue if any of these apply:
- You take an SSRI, SNRI, MAOI or other serotonergic medicine. Methylene blue is a potent inhibitor of the enzyme MAO-A, and combining it with serotonin-raising medicines can cause serotonin syndrome, a medical emergency (Ramsay 2007).
- You have G6PD deficiency, or don’t know your status and have a family history of it. A pharmacy review lists methylene blue among the medicines that should not be used until a G6PD test has been done (Belfield 2018).
- You are pregnant or breastfeeding.
- You are under 18.
Also expect your urine to turn blue or green. In the brain-imaging trial below, every participant who took methylene blue reported blue urine (Rodriguez 2016). It is an expected effect of the dye, but it can alarm people who aren’t expecting it. If you take any prescription medicine, talk to your doctor or pharmacist before starting methylene blue. Our methylene blue safety guide covers serotonin syndrome and MAO inhibition in more detail.
One more point for existing customers. Our Cellular Longevity Stack already contains NMN, so don’t add a second NMN product on top of it.
NMN and methylene blue: what each one does
NMN supplies the raw material for NAD+
NMN (nicotinamide mononucleotide) is a building block your cells turn into NAD+, a coenzyme used in energy metabolism and by enzymes such as sirtuins. Taking NMN by mouth raises NAD+ in the blood: in a 12-week trial of 42 healthy older men, 250mg a day raised blood NAD+ and related metabolites (Igarashi 2022), and in a 60-day trial of 80 healthy middle-aged adults, 300mg, 600mg and 900mg a day all raised blood NAD+ against placebo, with the highest levels at 600mg and 900mg (Yi 2023).
Methylene blue cycles electrons in the mitochondria
Methylene blue is a century-old dye and medicine. In cells, enzymes use NADH to turn it into a colourless reduced form, and cytochrome c in the mitochondria turns it back to blue, so it cycles between the two. In human fibroblasts (connective-tissue cells) grown in a lab, it increased mitochondrial complex IV activity by 30% and oxygen use by 37 to 70% (Atamna 2008). That is a cell-culture result. It shows a mechanism, not a benefit you will feel from a capsule.
Why people stack them
The idea behind the stack is simple. NMN raises the supply of NAD+, and methylene blue works on the electron chain that uses NADH. It makes sense on paper, which is why longevity clinics and biohackers talk about it. What it lacks is a trial: nobody has measured what the two do together in people, so any “synergy” is a hypothesis.
What the human studies show
NMN
- Blood NAD+: raised in both trials above, at 250mg to 900mg a day.
- Walking: in the 80-person trial, six-minute walking distance improved more than placebo at 300mg, 600mg and 900mg, with the longest distances at 600mg and 900mg (Yi 2023).
- Muscle: in the older men, gait speed and left-hand grip showed nominal improvements that the authors say need confirming in larger studies. Body composition did not change (Igarashi 2022).
- Insulin sensitivity: in a 10-week trial in postmenopausal women, 250mg a day increased muscle insulin sensitivity against placebo (Yoshino 2021). The trial was small.
Our NMN is 500mg per capsule, taken once a day. That sits inside the 250mg to 900mg range used in these trials. It is double the 250mg dose in the muscle and insulin studies and a little under the 600mg dose that did best on walking distance.
Methylene blue
The best-known human study is small. In a randomised, placebo-controlled brain-imaging trial, 26 healthy adults aged 22 to 62 took either a single 280mg dose of methylene blue (about 4mg per kg) or a placebo. An hour later the methylene blue group showed more brain activity during attention and memory tasks and 7% more correct answers in a memory test (Rodriguez 2016).
Be clear about the gap. Our capsule holds 10mg, which is 28 times less than the single dose in that trial. We found no published trial of 10mg a day of methylene blue for energy or focus. So the brain-imaging result tells you something about methylene blue at a much higher dose, once, in a lab. It does not tell you what a 10mg capsule does over weeks.
The stack itself has no human data at all. If you want to judge it for yourself, keep notes on sleep, energy and focus for a few weeks, and change one thing at a time.
The stack in numbers
Prices are from our store as of Sep 2026. Cost per day is the bottle price divided by the number of daily serves on the label.
| Product | Dose per daily serve | Bottle | Price (AUD, as of Sep 2026) | Cost per day | Buy |
|---|---|---|---|---|---|
| Eternal Elixir NMN 500mg | 500mg NMN (1 capsule) | 90 capsules, 90 days | $59.99 | $0.67 | NMN 500mg |
| Eternal Elixir Methylene Blue 10mg capsules | 10mg methylene blue (1 capsule) | 60 capsules, 60 days | $59.99 | $1.00 | Methylene Blue capsules |
| Eternal Elixir Methylene Blue Liquid 1% | 10mg per dropper | 50 serves | $49.99 | $1.00 | Methylene Blue drops |
| Vitasolutions NMN & Resveratrol (sold by us) | 1,100mg NMN and resveratrol combined (2 capsules; split not stated) | 60 capsules, 30 days | $49.99 | $1.67 | Vitasolutions |
Add NMN 500mg to cart · $59.99
NMN 500mg with the methylene blue capsules costs $1.67 a day. Swapping the capsules for the drops costs the same per day, but drops stain: capsules are swallowed, so they avoid the blue mouth and tongue that drops can leave. Because the NMN bottle lasts 90 days and the methylene blue bottle 60, you will reorder them at different times. Two NMN bottles save 10%, and orders over $100 ship free.
How to take NMN and methylene blue together
Follow each label. There is no trial-tested protocol for the combination, so what follows is the label directions plus some common sense.
- Check the safety list above first. If you take any serotonergic medicine, stop here and speak to your doctor.
- Start with one product. Take methylene blue alone for a week or two, then add NMN. If something disagrees with you, you will know which one caused it.
- NMN: one 500mg capsule a day. The label says preferably in the morning.
- Methylene blue: one 10mg capsule a day, as the label directs. Don’t take more than the label says.
- Taking both: once you know each suits you, you can take them together in the morning. Nothing in the research suggests they need to be spaced apart, and nothing shows that taking them together adds anything.
- Review after a few weeks. If you notice nothing after two months, you have your answer for your own body.
For drops, measuring and timing, see our guide on how to take methylene blue drops, and for choosing a format, methylene blue liquid vs capsules. If you drink coffee in the morning, our piece on methylene blue and coffee covers that pairing.
What not to mix with NMN
NMN interactions have not been studied in detail. A few practical points:
- Stay at 500mg a day. NMN products sold in Australian pharmacies carry label limits. Bioglan NMN 500 at Chemist Warehouse, for example, says to take one 500mg capsule daily and not to use it for more than 12 weeks. Our capsule is 500mg, one a day.
- Don’t double up. If you already take an NMN blend, a stack containing NMN, or an NAD+ drink, count its NMN before adding more.
- Pregnancy and medicines. Bioglan’s label says it is not recommended in pregnancy or breastfeeding. If you take prescription medicines, check with your doctor or pharmacist first.
For more on NMN itself, compare products in our best NMN supplements in Australia guide, or read our guide to NAD+ boosters. If you want a seasonal version of this stack, our pre-winter mitochondrial stack adds berberine. And if you’re weighing resveratrol against pterostilbene, see pterostilbene vs resveratrol.
Does methylene blue make you look younger?
There is no human trial showing that. The anti-ageing interest comes from lab and animal work, including studies of methylene blue on skin cells and skin models, summarised in a 2021 review whose senior author founded a methylene blue skincare company (Xue 2021). Those are laboratory and animal findings. They don’t show that a 10mg capsule changes how your skin looks, and we don’t claim it does.
Who this stack suits, and who it doesn’t
It suits healthy adults who take no serotonergic medicines, have no G6PD deficiency, and want to try both an NAD+ precursor at a trial-range dose and a low-dose methylene blue, while accepting that the combination is untested.
It doesn’t suit anyone on antidepressants or other serotonergic medicines, anyone with G6PD deficiency, anyone pregnant, breastfeeding or under 18, or anyone who wants a product backed by trials of that exact product. If you want the NAD+ side only, NMN 500mg on its own is the simpler choice. For a wider look at where methylene blue sits among focus supplements, see methylene blue vs other nootropics and our methylene blue guide. To compare methylene blue brands, see the best methylene blue supplements in Australia, or browse the full range.
Frequently asked questions
Can you take methylene blue and NMN at the same time?
No interaction between them has been reported, and no study has tested them together. If you are healthy and take no serotonergic medicines, taking one capsule of each in the morning follows both labels. Start one at a time first so you know how each affects you, and talk to your doctor if you take any prescription medicine.
Can you take NAD and methylene blue together?
NAD+ products (NAD+ itself, NMN or nicotinamide riboside) and methylene blue have no reported interaction, but the combination has not been studied. The rules that matter come from methylene blue: not with serotonergic medicines, not with G6PD deficiency, not in pregnancy or breastfeeding, and not under 18.
What not to mix with methylene blue?
Do not mix methylene blue with SSRIs, SNRIs, MAOIs or other medicines that raise serotonin, because methylene blue strongly inhibits MAO-A and the combination can cause serotonin syndrome. Don’t take it if you have G6PD deficiency. Don’t combine two methylene blue products, including a stack that already contains it. Ask your pharmacist to check any prescription medicine you take.
Add Methylene Blue capsules to cart · $59.99
What should you not mix NMN with?
There are no well-studied NMN drug interactions, but keep your total NMN to 500mg a day and count any NMN in blends or stacks you already take. Several Australian pharmacy NMN labels also say not to use it for more than 12 weeks and not in pregnancy or breastfeeding. Check with your doctor if you take prescription medicines.
How long before I notice anything?
The NMN trials measured changes after 6 to 12 weeks for muscle and 30 to 60 days for walking distance. The methylene blue brain-imaging effect was measured one hour after a single 280mg dose, which is not comparable to a 10mg capsule. Give the stack a couple of months and track how you feel.
Will methylene blue turn my urine blue?
Yes, blue or green urine is common. Every participant who took methylene blue in the brain-imaging trial reported blue urine. Drops can also stain your mouth and tongue, which capsules avoid.
What is the best NMN and methylene blue stack in Australia?
Eternal Elixir NMN 500mg with Eternal Elixir Methylene Blue 10mg capsules is our pick: one capsule of each a day, $1.67 a day in total, shipped from Sydney within 24 hours. We make both. NMN 500mg sits inside the dose range of the human NMN trials. The methylene blue dose is far below the single dose tested in the brain-imaging study, and the combination itself is untested.
Add NMN 500mg to cart · $59.99
The bottom line
Eternal Elixir NMN 500mg and Methylene Blue 10mg capsules are our stack: $1.67 a day for 500mg of NMN and 10mg of methylene blue. NMN in this dose range has human trials behind it for raising blood NAD+. Methylene blue at 10mg has none, and the pair has never been tested together. Read the safety list, start one product at a time, and speak to your doctor first if you take any prescription medicine.
Sources
- Ramsay RR, Dunford C, Gillman PK. Methylene blue and serotonin toxicity: inhibition of monoamine oxidase A (MAO A) confirms a theoretical prediction. Br J Pharmacol. 2007;152(6):946-951. PubMed 17721552
- Belfield KD, Tichy EM. Review and drug therapy implications of glucose-6-phosphate dehydrogenase deficiency. Am J Health Syst Pharm. 2018;75(3):97-104. PubMed 29305344
- Rodriguez P, Zhou W, Barrett DW, et al. Multimodal randomized functional MR imaging of the effects of methylene blue in the human brain. Radiology. 2016;281(2):516-526. PubMed 27351678
- Atamna H, Nguyen A, Schultz C, et al. Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways. FASEB J. 2008;22(3):703-712. PubMed 17928358
- Xue H, Thaivalappil A, Cao K. The potentials of methylene blue as an anti-aging drug. Cells. 2021;10(12):3379. PubMed 34943887
- Igarashi M, Nakagawa-Nagahama Y, Miura M, et al. Chronic nicotinamide mononucleotide supplementation elevates blood nicotinamide adenine dinucleotide levels and alters muscle function in healthy older men. NPJ Aging. 2022;8(1):5. PubMed 35927255
- Yi L, Maier AB, Tao R, et al. The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in healthy middle-aged adults: a randomized, multicenter, double-blind, placebo-controlled, parallel-group, dose-dependent clinical trial. GeroScience. 2023;45(1):29-43. PubMed 36482258
- Yoshino M, Yoshino J, Kayser BD, et al. Nicotinamide mononucleotide increases muscle insulin sensitivity in prediabetic women. Science. 2021;372(6547):1224-1229. PubMed 33888596
- Bioglan NMN 500mg 30 capsules, label directions and warnings, Chemist Warehouse, checked September 2026. chemistwarehouse.com.au
